FastQCFastQC Report
Sun 20 Nov 2022
CO_005-RNA.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_005-RNA.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65325
Sequences flagged as poor quality0
Sequence length25-356
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCACATCTGTCCAGT1061616.251052430156907No Hit
CCTGCTGCTCGTCCAGCTTCCGAAGCCGGGTGTTGAGGTTTCCCCGAATA734011.23612705702258No Hit
GCGAAGAGCAGCCCAGCTGCAGAGAAAGTTCCCGCATCTGGAGTTCAGGA733211.223880597014926No Hit
CACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTACCGCAGCTGC62429.555300420972063No Hit
GCCAGGAGCCTGCCTCTTTTCCACAGAAACAACATCGATTTCTTCCTCAT46467.1121316494450815No Hit
GAGGAGACACCGCCCACCACCAGCAGCGACTCTGAGGAGGAACAAGAAGA45506.965174129353234No Hit
TACTTAAGCAGGTACCAAACCCAGGCCCTCCTCCCCGCTGCCCATCTCCC40396.182931496364332No Hit
TACTTAAGCAGGTACCAAACCCAGGCCCTCCTCCCCGCTGCCCATCCTCC12141.8584003061615002No Hit
GCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTTGGTGGAGGAT11351.7374665135859164No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGCAGCTG9821.503252965939533No Hit
CCAACTACACGTTACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGAT7911.210868733256793No Hit
GACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTAC6771.036356678147723No Hit
GCTCCCGTTAAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCACAT6621.0133945656333716No Hit
GGCAAACCACGCGATATCTTGGCAAACCACGCAC5850.8955223880597015No Hit
GTGCGTGGTTTGCCAAGATATCGCGTGGTTTGCC5490.8404133180252583No Hit
TACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGATTTTGACTACCGA4900.7500956754688098No Hit
GGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTCATCCTCCACCAAG4250.6505931879066207No Hit
GCAATGGTCTTTAGGTGGGAACACTGT3550.543436662839648No Hit
CTGACCTTTTGCCAGGAGCCTGCCTCTTTTCCACAGAAACAACATCGATT3500.5357826253348641No Hit
CCAACTACACGTTACTTAAGCAGGTACCAAACCCAGGCCCTCCTCCCCGC2230.3413700727133563No Hit
GGGGTCTCGACTCTGAGCACGACCGTGCCTCTGGCGTCATCCTCCACCAA2200.336777650210486No Hit
GCTCCCTTTTCTTGGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTC2000.30616150019135097No Hit
AGCCACGGAACCTCCAAGAATGTTCTGTT1330.20359739762724838No Hit
CTTAGGATTGGCTGCAATCATGCACTTGGATTGGCTGC1280.1959433601224646No Hit
GCAATGGTCTTTAGGTCAAGTTTACAACCAAGATTCACTGTGGATACAAT1270.19441255262150786No Hit
AACAGAACATTCTTGGAGGTTCCGTGGCT1160.17757367011098354No Hit
GCGAAGAGCAGCCCAGCTGCAGAGAAAGTTCCCGCATCTGGGGTTCAGGA1060.162265595101416No Hit
GCAGCCAATCCAAGTGCATGATTGCAGCCAATCCTAAG1020.156142365097589No Hit
GGCACTGAACTCCTGCTGCTCGTCCAGCTTCCGAAGCCGGGTGTTGAGGT910.13930348258706468No Hit
GCCAGGAGCCTGCCTCTTTCCACAGAAACAACATCGATTTCTTCCTCATC890.13624186758515117No Hit
CCTGCTGCTCGTCCAGCTTCCGAAGCCGGGTGTTGAGGTTTCCCCCGAAT870.13318025258323765No Hit
GCGAAGAGCAGCCCAGCTGCAGAGAAAGTTCCCCGCATCTGGAGTTCAGG780.11940298507462686No Hit
AAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCCACATCTGTCCAG690.10562571756601608No Hit
GGCAAACCACGCGAATATCTTGGCAAACCACGCAC670.10256410256410256No Hit
GATGGCTGGTGCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTT660.10103329506314582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)